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Altogen Biosystems Provides Stable Xenograft Models


Altogen Biosystems is a ­global provider of transfection reagents and quality services for pharmaceutical companies, laboratories, and researchers involved in biotechnology-related fields. Biology contract research products that are offered include assay development, 28-day generation of specific stably-transfected cell lines, and other laboratory services like siRNA screening and ELISA assay development. For an AGS cell xenograft model, visit the Altogen Biosystems website.

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Effects of H2O2 on the Proliferation of AGS Cells

In the following study, researchers investigate the effect of low concentration hydrogen peroxide (H2O2) on the proliferation of AGS cells. AGS cells were treated with low concentrations of H2O2 for 48 hours. Methyl thiazolyl tetrazolium (MTT) method was used to analyze the effect of H2O2 concentration gradient on the activity of AGS cells. Western blot analysis was used to analyze the expression of epidermal growth factor receptor (EGFR) and its downstream signaling pathway extracellular signal-regulated kinase (ERK) protein in H2O2. Results show that after applying low concentration of H2O2 treatment on AGS cells for 48 hours, EGFR protein levels and ERK protein phosphorylation levels increased significantly. These results suggest that low concentration of H2O2 can significantly increase the proliferation of AGS cells by EGFR/ERK signaling pathway. [ LINK ]
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AGS Cell Line

The AGS cell line originated in 1979 from the stomach tissue of a 54-year-old Caucasian female who had been diagnosed with gastric adenocarcinoma. AGS cells are tumorigenic, hyperdiploid, and have a modal chromosome number of 49. AGS exhibits adherent cultural properties and has an epithelial morphology. The AGS cell line is a good host for the study of stomach cancer and other stomach infections using  in vivo and in vitro  transfection methods. An AGS Transfection Reagent to transfect AGS cells is commercially available from Altogen Biosystems , and an AGS xenograft murine model is displayed  here .
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AGS Transfection Reagent

In 1979 the AGS cell line (ATTC number CRL 1739) was established from a fragment tumor of a Causcasian female patient with gastric adenocarcinoma (stomach cancer). Without prior treatment to the tumorigenic cells they were extracted from the patient. These cells have been described as exhibiting   adherent growth properties as well as epithelial cellular morphology. Using siRNA transfection, a 2015 study published in the International Journal of Oncology proved that the inhibition of HDAC (histone deacetylase) was discovered to suppress the growth of gastric adenocarcinoma cells.  The use of biodegradable polymer based transfection agents, such as those available through  Altogen Biosystems  minimize toxicity and inflammatory responses. Following entry into the cell, the reagent will facilitate release of the transgene (siRNA, shRNA, miRNA, mRNA and plasmid DNA), and will naturally degrade. The AGS transfection reagent increases siRNA uptake with little to no loss...
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