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Effects of H2O2 on the Proliferation of AGS Cells

In the following study, researchers investigate the effect of low concentration hydrogen peroxide (H2O2) on the proliferation of AGS cells. AGS cells were treated with low concentrations of H2O2 for 48 hours. Methyl thiazolyl tetrazolium (MTT) method was used to analyze the effect of H2O2 concentration gradient on the activity of AGS cells. Western blot analysis was used to analyze the expression of epidermal growth factor receptor (EGFR) and its downstream signaling pathway extracellular signal-regulated kinase (ERK) protein in H2O2. Results show that after applying low concentration of H2O2 treatment on AGS cells for 48 hours, EGFR protein levels and ERK protein phosphorylation levels increased significantly. These results suggest that low concentration of H2O2 can significantly increase the proliferation of AGS cells by EGFR/ERK signaling pathway. [LINK]

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