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AGS Transfection Reagent

In 1979 the AGS cell line (ATTC number CRL 1739) was established from a fragment tumor of a Causcasian female patient with gastric adenocarcinoma (stomach cancer). Without prior treatment to the tumorigenic cells they were extracted from the patient. These cells have been described as exhibiting  adherent growth properties as well as epithelial cellular morphology. Using siRNA transfection, a 2015 study published in the International Journal of Oncology proved that the inhibition of HDAC (histone deacetylase) was discovered to suppress the growth of gastric adenocarcinoma cells. 

 The use of biodegradable polymer based transfection agents, such as those available through Altogen Biosystems minimize toxicity and inflammatory responses. Following entry into the cell, the reagent will facilitate release of the transgene (siRNA, shRNA, miRNA, mRNA and plasmid DNA), and will naturally degrade. The AGS transfection reagent increases siRNA uptake with little to no loss of cell viability. This less harsh reagent can even be used for single cell analysis, with a 77% transfection efficacy. 
Transfection Reagent for AGS Cells (Gastric Adenocarcinoma, CRL1739)
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AGS Cell Line

The AGS cell line originated in 1979 from the stomach tissue of a 54-year-old Caucasian female who had been diagnosed with gastric adenocarcinoma. AGS cells are tumorigenic, hyperdiploid, and have a modal chromosome number of 49. AGS exhibits adherent cultural properties and has an epithelial morphology. The AGS cell line is a good host for the study of stomach cancer and other stomach infections using  in vivo and in vitro  transfection methods. An AGS Transfection Reagent to transfect AGS cells is commercially available from Altogen Biosystems , and an AGS xenograft murine model is displayed  here .
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