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AGS Cells - Transfection and Xenografting Applications

AGS cells are derived from the gastric tissue of a 54-year-old female, and represent one of the major players in the field of genomics research in gastric cancer. The cells are suitable for preclinical xenografts, and are prime subjects for transfection experiments. AGS cells have been researched extensively for pharmaceutical applications of histone deacetylase inhibition in stalling the growth of gastric cancer. They can easily serve as recipients of transfected genomic material, such as siRNA, which can be used for library screenings and the development of gene therapies specific to gastric cancer.

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AGS Cell Line

The AGS cell line originated in 1979 from the stomach tissue of a 54-year-old Caucasian female who had been diagnosed with gastric adenocarcinoma. AGS cells are tumorigenic, hyperdiploid, and have a modal chromosome number of 49. AGS exhibits adherent cultural properties and has an epithelial morphology. The AGS cell line is a good host for the study of stomach cancer and other stomach infections using  in vivo and in vitro  transfection methods. An AGS Transfection Reagent to transfect AGS cells is commercially available from Altogen Biosystems , and an AGS xenograft murine model is displayed  here .
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Effects of H2O2 on the Proliferation of AGS Cells

In the following study, researchers investigate the effect of low concentration hydrogen peroxide (H2O2) on the proliferation of AGS cells. AGS cells were treated with low concentrations of H2O2 for 48 hours. Methyl thiazolyl tetrazolium (MTT) method was used to analyze the effect of H2O2 concentration gradient on the activity of AGS cells. Western blot analysis was used to analyze the expression of epidermal growth factor receptor (EGFR) and its downstream signaling pathway extracellular signal-regulated kinase (ERK) protein in H2O2. Results show that after applying low concentration of H2O2 treatment on AGS cells for 48 hours, EGFR protein levels and ERK protein phosphorylation levels increased significantly. These results suggest that low concentration of H2O2 can significantly increase the proliferation of AGS cells by EGFR/ERK signaling pathway. [ LINK ]
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