AGS Transfection Reagent (Gastic Adenocarcinoma, CRL 1739)

AGS cells are derived from the gastric tissue of a 54-year-old female, and represent one of the major players in the field of genomics research in gastric cancer. The cells are suitable for preclinical xenografts , and are prime subjects for transfection experiments. AGS cells have been researched extensively for pharmaceutical applications of histone deacetylase inhibition in stalling the growth of gastric cancer. They can easily serve as recipients of transfected genomic material, such as siRNA, which can be used for library screenings and the development of gene therapies specific to gastric cancer.

Altogen Biosystems is a ­global provider of transfection reagents and quality services for pharmaceutical companies, laboratories, and researchers involved in biotechnology-related fields. Biology contract research products that are offered include assay development, 28-day generation of specific stably-transfected cell lines, and other laboratory services like siRNA screening and ELISA assay development. For an AGS cell xenograft model, visit the Altogen Biosystems website.

In 1979 the AGS cell line (ATTC number CRL 1739) was established from a fragment tumor of a Causcasian female patient with gastric adenocarcinoma (stomach cancer). Without prior treatment to the tumorigenic cells they were extracted from the patient. These cells have been described as exhibiting   adherent growth properties as well as epithelial cellular morphology. Using siRNA transfection, a 2015 study published in the International Journal of Oncology proved that the inhibition of HDAC (histone deacetylase) was discovered to suppress the growth of gastric adenocarcinoma cells.  The use of biodegradable polymer based transfection agents, such as those available through  Altogen Biosystems  minimize toxicity and inflammatory responses. Following entry into the cell, the reagent will facilitate release of the transgene (siRNA, shRNA, miRNA, mRNA and plasmid DNA), and will naturally degrade. The AGS transfection reagent increases siRNA uptake with little to no loss...